Analyzing Peptidoglycan Elasticity of E. coli Using Hyperosmotic Shock Studies
Type of Presentation
Presentation
Type of Project
Project I/II
Presenter 1 Program
INSC
Presenter 1 Location
Harrisburg University of Science and Technology
Project Description
This study investigated the physical properties of Escherichia coli cells grown in different media and subjected to various hyperosmotic shock conditions. Image analysis at 100X was used to measure changes in cell length, and the results showed a statistically significant difference between the media types, conditions, and their interaction. The study found that as the growth rate increases, the cell becomes relatively less stiff, and conversely, as the growth rate decreases, the cell becomes relatively stiffer. The study suggests that the cross-link density and relative stiffness of the cell are closely related to the growth rate and can influence the cell's response to osmotic stress. The findings provide valuable insight into the mechanisms underlying bacterial cell wall elasticity, osmoregulation, and antibiotic resistance. Future studies could increase the concentration of hyperosmotic shock conditions and develop the Zetasizer method to accurately reflect the average cell length of bacteria present in the samples.
Faculty Member
Akeisha Belgrave, PhD
Analyzing Peptidoglycan Elasticity of E. coli Using Hyperosmotic Shock Studies
This study investigated the physical properties of Escherichia coli cells grown in different media and subjected to various hyperosmotic shock conditions. Image analysis at 100X was used to measure changes in cell length, and the results showed a statistically significant difference between the media types, conditions, and their interaction. The study found that as the growth rate increases, the cell becomes relatively less stiff, and conversely, as the growth rate decreases, the cell becomes relatively stiffer. The study suggests that the cross-link density and relative stiffness of the cell are closely related to the growth rate and can influence the cell's response to osmotic stress. The findings provide valuable insight into the mechanisms underlying bacterial cell wall elasticity, osmoregulation, and antibiotic resistance. Future studies could increase the concentration of hyperosmotic shock conditions and develop the Zetasizer method to accurately reflect the average cell length of bacteria present in the samples.