Presenter Information

Rebecca ConnerFollow

Type of Presentation

Presentation

Type of Project

Project I/II

Presenter 1 Program

INSC

Presenter 1 Location

Harrisburg University of Science and Technology- Harrisburg Campus

Project Description

Vitamin D3 is an important chemical in the human body, however, many Americans have low levels of this vitamin. There are plenty of oral supplementations for Vitamin D3 deficiency, but those of older age and busy schedules may struggle to meet the minimum requirement. A recently developed ethosomal transmembrane delivery system (Touitou, 2000), similar to liposomes but also containing ethanol, allows users to apply a gel dermally and have the desired drug or active ingredient reach the bloodstream faster. However, there is considerable variation in the concentration of ethanol and permeation enhancers used. Using an affordable method to mimic skin diffusion, this study examined Vitamin D3-containing ethosomes formed using varying concentrations of ethanol and propylene glycol to find the ideal ratio to allow the maximum amount of Vitamin D3 to diffuse through a model membrane. Using spectroscopic analysis, it was found that a ratio of XX:XX of ethanol to propylene glycol yielded the most effective transfer of Vitamin D3 across the model membrane. Further analysis of Vitamin D3 concentration will be carried out using high-performance liquid chromatography to verify the concentrations passed through. The effectiveness of the ethosomes will also be tested on other model membranes, such as pig ear skin, that mimic the biochemistry of human skin more closely.

Faculty Member

Andrea Nagy

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Determining the Ideal Concentrations of Ethanol and Propylene Glycol in Ethosomes for Transdermal Delivery of Vitamin D3

Vitamin D3 is an important chemical in the human body, however, many Americans have low levels of this vitamin. There are plenty of oral supplementations for Vitamin D3 deficiency, but those of older age and busy schedules may struggle to meet the minimum requirement. A recently developed ethosomal transmembrane delivery system (Touitou, 2000), similar to liposomes but also containing ethanol, allows users to apply a gel dermally and have the desired drug or active ingredient reach the bloodstream faster. However, there is considerable variation in the concentration of ethanol and permeation enhancers used. Using an affordable method to mimic skin diffusion, this study examined Vitamin D3-containing ethosomes formed using varying concentrations of ethanol and propylene glycol to find the ideal ratio to allow the maximum amount of Vitamin D3 to diffuse through a model membrane. Using spectroscopic analysis, it was found that a ratio of XX:XX of ethanol to propylene glycol yielded the most effective transfer of Vitamin D3 across the model membrane. Further analysis of Vitamin D3 concentration will be carried out using high-performance liquid chromatography to verify the concentrations passed through. The effectiveness of the ethosomes will also be tested on other model membranes, such as pig ear skin, that mimic the biochemistry of human skin more closely.