Type of Presentation
Poster
Type of Project
Class Project
Project Description
Liposomes were synthesized using the thin film method. A lyophilized power of egg-derived phosphatidylcholine, stearylamine, and cholesterol were added to ethanol and dried under argon to form a lipid cake. The lipid cake was rehydrated with dPBS and sonicated at 60°C forming a heterogenous batch of liposomes. Our results revealed the average size of the liposomes, determined by Dynamic Light Scattering, was approximately 223.1nm, while demonstrating a weakly positive zeta-potential of 1.9± 8.07mv. Next, we tested the antitumor action of the liposomes in the HEK-293 cell line via an MTT assay. We observed that the liposomes were able to inhibit cell proliferation in the HEK-293 cell line in comparison to controls. Future studies will focus on encapsulating hydrophobic and hydrophilic molecules within the liposome formulation to improve delivery to cancer cells.
Faculty Member
Christine Skibinski PhD
Included in
Biotechnology Commons, Medical Biotechnology Commons, Nanotechnology Commons, Oncology Commons, Pharmacy and Pharmaceutical Sciences Commons, Research Methods in Life Sciences Commons
Liposome Synthesis and Evaluation in the HEK-293 Cell Line
Liposomes were synthesized using the thin film method. A lyophilized power of egg-derived phosphatidylcholine, stearylamine, and cholesterol were added to ethanol and dried under argon to form a lipid cake. The lipid cake was rehydrated with dPBS and sonicated at 60°C forming a heterogenous batch of liposomes. Our results revealed the average size of the liposomes, determined by Dynamic Light Scattering, was approximately 223.1nm, while demonstrating a weakly positive zeta-potential of 1.9± 8.07mv. Next, we tested the antitumor action of the liposomes in the HEK-293 cell line via an MTT assay. We observed that the liposomes were able to inhibit cell proliferation in the HEK-293 cell line in comparison to controls. Future studies will focus on encapsulating hydrophobic and hydrophilic molecules within the liposome formulation to improve delivery to cancer cells.