Completion Date

4-23-2023

Document Type

Thesis

Degree Name

Master of Science (MS)

Program or Discipline Name

Biotechnology

First Advisor

Mrunalini Pattarkine

Abstract

Numerous scientific research studies have focused on the significance of mRNA stability and its influence on gene expression and translation. The stability of mRNA has been extensively studied in relation to gene expression, with recent research and advancements in high-throughput RNA sequencing methods indicating that mutations and modifications to the nucleotide sequence of mRNA can significantly impact its regulation. The molecular mechanisms regulating the stability of human GLP-1 mRNA through nucleotide modifications and their effects on GLP-1 protein therapeutics have been studied and characterized in various in-vitro and in-vivo studies to screen various GLP-1 receptor agonists (GLP-1 RAs) that have shown to increase GLP-1 mRNA stability by achieving greater mRNA half-life, prolonged expression, and decreased endogenous cleavage by DPP-IV protease. This review paper seeks to provide a comprehensive understanding of few GLP-1 receptor agonists - their half-life and stability, in-vitro and in-vivo characterization studies, various clinical studies and case studies, which could inform the development of more effective GLP-1 protein therapeutics for Type 2 diabetes mellitus (T2DM) therapy.

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