Faculty Works
Document Type
Article
Publication Date
11-28-2000
Abstract
The hybridization of DNA by sequential electrostatic and hydrogen-bonding immobilization of single-stranded complementary oligonucleotides at the air-water interface with cationic Langmuir monolayers is demonstrated. The complexation of the single-stranded DNA molecules with octadecylamine (ODA) Langmuir monolayers was followed in time by monitoring the pressure-area isotherms. A large (and slow) expansion of the ODA monolayer was observed during each stage of complexation in the following sequence: primary single-stranded DNA followed by complementary single-stranded DNA followed by the intercalator, ethidium bromide. Langmuir-Blodgett (LB) films of the ODA-DNA complex were formed on different substrates and characterized using quartz-crystal microgravimetry (QCM), Fourier transform infrared spectroscopy (FTIR), polarized-UV-vis and fluorescence spectroscopy, as well as thermal melting studies. These measurements clearly showed that hybridization of the complementary single-stranded DNA molecules had occurred at the air-water interface, leading to the characteristic double-helical structure. Furthermore, it was observed that the DNA molecules in the LB films were oriented parallel to the substrate withdrawal direction.
Recommended Citation
Sastry, M., Ramakrishnan, V., Pattarkine, M., Gole, A., & Ganesh, K.
(2000). Hybridization of DNA by sequential immobilization of oligonucleotides at the air-water interface. Langmuir, 16 (24), 9142-9146.
Publication Title
Langmuir
Start Page No.
9142
End Page No.
9146
ISSN
1520-5827
DOI
10.1021/la000753z
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