Faculty Works
Document Type
Article
Publication Date
6-1-1996
Abstract
Purple membrane (PM, λ" role="presentation">λmax" role="presentation">max 570 nm) of H. halobium on treatment with sulphuric acid changes its colour to blue (λ" role="presentation">λmax" role="presentation">max 608 nm). The purple chromophore can be regenerated from the blue chromophore by exogeneous addition of anions such as CI−" role="presentation">− and HPO42−" role="presentation">2−4. Chloride ion is found to be more effective than the dibasic phosphate ion in regenerating the purple chromophore. Nevertheless, one thing common to the anion regeneration is that both CI−" role="presentation">− and HPO42−" role="presentation">2−4 show marked pH effect. At pH 1.0 the efficiency of regeneration of the purple chromophore is greater than at pH 2.0, for the same anion concentration. Fluorescence and circular dichroic studies indicate that the proteins do not undergo drastic changes at the secondary' or tertiary structure level and the native structure is preserved during this transition. However, chromophoric-site interactions between retinal and the apoprotein are affected during this colour transition. A molecular mechanism is advanced for this transition.
Recommended Citation
Pattarkine, M., & Singh, A. K. (1996). ANION INDUCED BLUE TO PURPLE TRANSITION IN BACTERIORHODOPSIN. Journal of Photoscience, 3 (2), 71-76. Retrieved from https://digitalcommons.harrisburgu.edu/biotechnology_faculty-works/11
Publication Title
Journal of Photoscience
Start Page No.
71
End Page No.
76
ISSN
1225-8555
Included in
Analytical, Diagnostic and Therapeutic Techniques and Equipment Commons, Biochemical and Biomolecular Engineering Commons, Biology and Biomimetic Materials Commons, Biomaterials Commons, Biomedical Commons, Bioresource and Agricultural Engineering Commons, Biotechnology Commons, Business Administration, Management, and Operations Commons, Computational Biology Commons, Food Biotechnology Commons, Food Microbiology Commons, Food Processing Commons, Health Economics Commons, Medical Biotechnology Commons, Medical Molecular Biology Commons, Medical Pharmacology Commons, Molecular Biology Commons, Molecular, Cellular, and Tissue Engineering Commons, Molecular, Genetic, and Biochemical Nutrition Commons, Molecular Genetics Commons, Nanomedicine Commons, Nanoscience and Nanotechnology Commons, Nanotechnology Fabrication Commons, Pharmacy and Pharmaceutical Sciences Commons